Team: Dynamics of Structure and Interactions of Macromolecules in Biology (DSIMB)
DSIMB is a bioinformatics team in Inserm unit UMR-S1134 (former UMR-S665), whose focus is the study of membrane proteins involved in red blood cell. The long-term scientific goal of DSIMB is to elucidate the mechanisms governing important functions of proteins of the RBC membrane, using information from protein structure and dynamics. Both membrane protein structure and dynamics determine RBC function, but they are difficult to characterize experimentally. Our expertise in structural bioinformatics and molecular simulations enables us to tackle both aspects: the prediction of 3D protein structures and the exploration of their dynamics.
Part of our activity is devoted to the development of methodologies to characterize membrane systems. These methodologies aim at predicting structural and functional properties of membrane proteins from sequence and at elucidating dynamics and thermodynamics properties starting from 3D structures. We also apply those methodologies to specific cases of interest for our unit and for GR-Ex. We consider with particular interest systems relevant for diseases studied by GR-Ex members, and we plan to contribute to the design of drugs able to interfere with the disease mechanisms.
Our team has contributed to:
- The development of bioinformatics tools for predicting structural and dynamics properties of proteins. Tools are available on www.dsimb.inserm.fr
- A better understanding of Duffy Antigen/Receptor for Chemokines (DARC): These chemokine receptors are erythrocyte transmembrane receptors for Plasmodium knowlesi and Plasmodium vivax merozoites, two major agents of malaria. We elaborated 3D structural models for DARC using experimental data provided by partner 2. These models were further used to predict potential binding zones between DARC and CXCL8 natural ligand and P. vivax partners. Once identified, these zones could be targeted with new designed molecules preventing interaction.
- DARC VHHs: We modelled the structure of VHH sequences able to bind Nterminus of DARC and we identified important amino acids involved in VHH binding. This work was conducted in collaboration with O. Bertrand (Team 1) and Dr. Mr. Czerwinski, Wroclaw, Poland, on camel VHHs. This model system allows the development of new tools in transfusion medicine (agglutination tests in routine) and could serve as blocking agents against P. vivax and HIV.
We will study transporter proteins in red blood cells with the aim at providing an insight into substrates transport mechanisms, understanding inhibition and predicting impact of mutations involved in pathological diseases, at an atomistic level. We will explore adhesion proteins in RBC in particular, integrins for gaining a molecular view on pathological cell adhesion to design anti-adhesive molecules.